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Homeostatic GAMMAdelta T-Cell Contents are Preserved by Granulocyte Colony-Stimulating Factor Priming and Correlate with the Early Recovery of GAMMAdelta T Subsets after Haploidentical Hematopoietic Transplantation
Bian, Z., Xu, L. P., Fu, Q., Huo, M., Liu, L., Zhao, X., Huang, X. J., Liu, J.
Biology of Blood & Marrow Transplantation. 2017
Abstract
Emerging evidence from graft manipulations and immunotherapeutic treatments has highlighted a favorable effect of gammadelta T cells in the setting of allogeneic hematopoietic stem cell transplantation (alloHSCT). However, gammadelta T subsets and their distinct features in the allograft have not been characterized. Additionally, whether homeostatic gammadelta T-cell fractions are influenced by treatment with granulocyte colony-stimulating factor (G-CSF) remains elusive. We initially compared the phenotypes of gammadelta T subsets including CD27+, CD27-, Vdelta1+, Vdelta2+, Vdelta1+CD27+, Vdelta1+CD27-, Vdelta2+CD27+, and Vdelta2+CD27- cells, in the peripheral blood of 20 healthy donors before and after G-CSF mobilization. The effects of G-CSF on the cytokine-production capacities of gammadelta T subsets were also detected. Moreover, the correlation between donor homeostatic gammadelta T-cell content and the early recoveries of gammadelta T subgroups after haploidentical HSCT was investigated in 40 pairs of donors and recipients. We found that both the proportions and IFN-gamma secretion capacities of peripheral gammadelta T subsets were preserved in G-CSF-primed grafts. Homeostatic Vdelta1 and Vdelta2 proportions of donors significantly correlated with the early recoveries of Vdelta1 and Vdelta2 cells after haploidentical HSCT. Interestingly, a higher day-30 Vdelta1 concentration was associated with a lower incidence of CMV reactivation in recipients. These results not only clarify the preservation of gammadelta T phenotypes and functional features by G-CSF mobilization, but also suggest the importance of homeostatic gammadelta T-cell content for immune recovery following alloHSCT. Copyright © 2017. Published by Elsevier Inc.