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Differences in cellular composition of peripheral blood stem cell grafts from healthy stem cell donors mobilized with either G-CSF alone or G-CSF and Plerixafor
Teipel, R., Oelschlagel, U., Wetzko, K., Schmiedgen, M., Kramer, M., Rucker-Braun, E., Holig, K., von Bonin, M., Heidrich, K., Fuchs, A., et al
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 2018
Abstract
This study was conducted to characterize and compare peripheral blood stem cell grafts from healthy donors, who underwent G-CSF-mobilization and subsequently received one dose of plerixafor, after insufficient stem cell yields were achieved at the first apheresis. Aliquots from 35 donors were collected from the first apheresis after mobilization with G-CSF alone, and from the second apheresis after additional plerixafor administration. Samples were freshly analyzed for cellular subsets by 8-color flow-cytometry. Leukapheresis samples mobilized with additional plerixafor showed a significant increase of total nucleated cells, including B cells, CD4(+) and CD8(+) T cells, and CD34(+) hematopoietic stem and progenitor cells. Absolute numbers of plasmacytoid dendritic cells were also significantly increased, whereas no changes were detected for myeloid dendritic cells. Furthermore, absolute numbers of regulatory T cells increased, with naive CD45RA(+) regulatory T cells showing the highest rise. Finally, strikingly higher numbers of myeloid derived suppressor cells were detected in the plerixafor and G-CSF mobilized graft. The mobilization of peripheral stem cells in healthy donors with G-CSF and plerixafor led to a significant difference in cellular graft composition compared to G-CSF alone. The clinical impact of the different cell composition for the graft recipient warrants further clinical investigation.